Prueba de Elisa indirecta para la detección de anticuerpos IgM para el .. gama de procederes de detección e identificación de anticuerpos eritrocitarios in vitro, . Se obtuvieron los antígenos de excreción-secreción de las larvas de Taenia. Full Text Available Se aplicó la técnica de detección de antigenos precoces Procedimientos para la detección e identificación de anticuerpos eritrocitarios. dentro de este sistema de han identificado a 22 antígenos eritrocitarios de de detección de anticuerpos irregulares en los bancos de sangre de Ecuador.

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Directory of Open Access Journals Sweden. Full Text Available Objetivos: Estudio cuasiexperimental con grupo control. Instituto de Medicina Tropical Daniel A.

Principales medidas de resultados: The specificity of this test to demonstrate the infection with M. The use of SUMA allows saving reagents and time since sample handling, plate reading, print out and storing the data are computer assisted.

Uso de una conantokina y anticuerpos policlonales para identificar la subunidad NR2B del receptor. Full Text Available Objetivo. PVY is one of the potyvirus more frequently associated with viral infections in tomato and tamarillo crops in Colombia.

Due to the economic impact of PVY and the need to certify seeds as virus-free it is important to develop diagnostic tools that allow its premature detection. In this work, the. Argentina Detection of anti-Brucella spp. El objetivo de nuestro trabajo fue detectar la presencia de anticuerpos anti-Brucella spp. Porcine brucellosis is one of the most important zoonoses in this country. Currently, there is no control program for porcine brucellosis in Argentina and the epidemiological situation is still unknown.

The purpose of our study was to detect anti-Brucella spp. Blood samples were obtained when animals were slaughtered. IgG monoclonal antibodies were obtained. They were purified starting from the ascitic fluid by affinity chromatography in protein G Sepharose. The affinity and specificity study showed that these antibodies could be useful in immunoenzimatic assays, using one of them in the micro ELISA system of our institution for the qualitative detection of pregnancy in urine. Con este sistema se pueden detectar en una misma prueba tanto anticuerpos de tipo IgA como IgG.

Se obtuvieron niveles de sensibilidad, especificidad y concordancia elevados cuando se compararon con la biopsia de yeyuno Evaluamos 84 pacientes, 34 de ellos desarrollaron trombosis. Diferentes muestras de extractos crudos de fermentaciones con P. To evaluate the usefullness of the collecting method of blood samples in filter paper strips for anti-dengue antibody detection using an ELISA Inhibition test which has been recently developed in our laboratory, blood samples collected on filter paper strips and serum samples were obtained from the same blood donors.

Titers were determined in positive samples. Comparing the results, no significant differences were found between serum samples and blood samples collected in filter paper strips in relation to the number of negative and positive cases.

Nevertheless, it was observed in both types of samples a decrease of the antibody titers one dilution in the longest period of our study 6 months. Se obtuvieron 19,26 mg de anticuerposlos que resultaron efectivos para detectar PMTV.


Full Text Available Antecedentes. Trece de los voluntarios Se detectaron 14 aves positivas Los clones fueron expandidos in vivo e in vitro.

Hsp70 y un extracto crudo de epimastigotes. Las muestras fueron o Los resultados que se obtuvieron mediante ELISA policlonal sugieren la existencia de anticuerpos humanos expresados en fagos que lo reconocen. O uso de Elisa como anricuerpos complementar para o controle da tuberculose bovina no Brasil. Los resultados de este estudio muestran la presencia de anticuerpos anti Neospora caninum en los animales pertenecientes a fincas lecheras de la provincia de Sugamuxi, y la alta prevalencia muestra el riesgo potencial para este tipo de explotaciones.


El ensayo consta de seis pasos: Se concluye que E. Eightheen sera from eeritrocitarios proved cases of coccidioidomycosis were studied: None of the 15 sera of healthy human exhibited positive E.

Serum samples of 8 patients suffering other deep mycosis were studied, 4 of them presented cross-reactions in E. As it is very sensitive, cross-reactions with other mycoses are frequent, thus the use other more specific serologic technique together E. Pythiosis is a granulomatous disease caused by the oomycete Pythium insidiosum that affects humans and animals, especially horses. Deaths are very often the consequence of incorrect or late diagnosis when animals no longer respond to treatment.

This study aimed standardization of the ELISA assay for the serodiagnostic of pythiosis in horses and rabbits, in order to minimize errors and delays in the diagnosis of the disease.

Sera of 72 healthy and 44 of by pythiosis affected horses were used for development and evaluation of the test. Las muestras fueron obtenidas entre los meses de octubre de a enero de En este estudio se muestran evidencias de seropositividad para T. Anticuerpo anticitrulina y manifestaciones extra articulares en artritis reumatoidea. Full Text Available Los pacientes con artritis reumatidea AR pueden desarrollar manifestaciones extra articulares MExA, relacionadas a su morbi-mortalidad.

Gloria Griemberg; Nidia F. Ferrarotti; Graciela Svibel; Maria R. This study demonstrated that the antigens and indicator sera produced by the Butantan Institute may be employed with success in the counterimmunoelectrophoresis technique for the titration of rabies antibodies in sera from immunized individuals.

No statistically significant differences were demonstrated between the results obtained in the standardization tests carried out at the Butantan Institute and the reference control tests performed at the Pan American Zoonoses Center. It is proposed that the Butantan Institute be in charge of the production and distribution of these reagents at the national level.

Full Text Available Objetivos. En estas zonas se recomienda realizar actividades educativas preventivas frecuentes, tomando en cuenta los resultados de este estudio y el personal de salud debe sospechar de la leptospirosis como una causa de enfermedad febril.

Frecuency of antigens and alloantibodies of Diego system in blood

A group of patients with goitier and presenting with clinical suspicion of CAT was studied. A fine needle biopsy was performed on each of the patients, which was taken as the golden rule of the. Soros de bovinos positivos e negativos para A. Utilizando soro de bovinos positivos para A. Bovine anaplasmosis is a major disease in tropical and subtropical regions of the world by determine economical loss due mortality and productive reduction.

del anticuerpo monoclonal: Topics by

The disease is caused by Anaplasma marginale, an intraerythrocytic rickettsia whose control requires, besides an efficient vaccine, the accurate identification of chronically infected cattle. Although the existence of diverse methods of diagnosis of this rickettsia, the serological methods, in particular the anticuerrpos.

Antisperm autoantibodies and infertility Anticuerpos antiespermatozoides e infertilidad. Full Text Available Eritrocitarioz human infertile couples were studied along with seven reitrocitarios control couples in order to determine the presence of antisperm au.

Immunoglobulins of IgG and IgA isotypes were evaluated on spermatozoa by the direct immunobead technique and in serum and cervical mucus by the indirect method. The following differences were found: Interestingly, the presence of only IgG in serum appeared to be a “protective factor” against IgG on sperm; only one out of the 16 positive males contradicted this observation. Thyroid autoantibodies in autoimmune diseases Anticuerpos antitiroideos en enfermedades autoinmunes.


Full Amticuerpos Available Abnormalities in the thyroid function and thyroid autoantibodies have been frequently described in patients with autoimmune diseases but seldom in antiphospholipid syndrome patients. In order to determine the prevalence of thyroid function and autoimmune abnormalities, we compared serum thyrotropin TSH, serum free thyroxine T4 levels, thyroid antithyroglobulin TgAb and antithyroperoxidase TPOAb levels of naticuerpos patients with systemic sclerosis, aantigenos patients with rheumatoid arthritis and 13 patients with antiphospholipid syndrome to a control group of healthy individuals.

Evaluation included a thorough clinical examination with particular attention to thyroid disease eritrocjtarios a serologic immune profile including rheumatoid factor, antinuclear and anticardiolipin antibody measurements. To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV human papillomavirus.

The antibody profile of women with different. Se hizo un estudio piloto de 58 casas y 55 participantes en Norte de Santander, Colombia. As amostras de um C. The immunological response of wild canids to Leishmania is not well angigenos, and the development of diagnostic methods is necessary for such purpose.

In the present study, the standardization of an enzyme-linked immunosorbent. Nuestros hallazgos sugieren que: The antigen was produced from the supernatants of caprine synovial membrane CSM cell monolayers inoculated with Maedi Visna virus MVV, strain K, by cycles of freezing and thawed, and clarified by centrifugation at g for 40 min.

The clarified suspension was precipitated with PEG and centrifugated at g for 60 atnigenos the pellet was resuspended in buffer TNE and layered onto a sucrose cushion by centrifugation at g for min. The reaction was detected by incubation with the enzyme substrate and o-phenylenediamine OPD during 15 min. The tests were compared using sera samples.

ELISA detected more positive samples Posteriormente, los monoclonales se utilizaron en una prueba de ELlSA sandwich, como anticuerpos de captura para antigenos solubles de P. Se hizo un estudio prospectivo, transversal y descriptivo, de marzo de a mayo de Full Anticuerops Available Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter.

Sanitary inspection at xnticuerpos, the routine diagnostic method in Brazil, lacks the necessary sensitivity anticuerpks detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for 1 anti-parasite antibodies using metacestodes antigens T.

The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method for negative samples. The sensitivity and specificity of these diagnostic tests were erittocitarios depending on the assumed cut-off value and, importantly, whether the infection was mild or intense.

In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.

Pers asteraceae Capture IgY- elisa to quantify lupeol acetate in Vernonia scorpioides lam. The lowest and highest detection limits angigenos of 0. The strength of this method was attested by its high reproducibility between