2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Cytology Osmosis and Water relations. Registration Forgot your password?

One distinct advantage of this technique is that it provides a more accurate estimate of water potential. Cut them to the same length with a razor blade ca.

Plant Physiology

Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. Examine the cores as you weigh them. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. My presentations Profile Feedback Log out. OK Any substance that can change shape or flow easily.

Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference. Published by Sabrina Grant Modified over 2 years ago. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data.

Chardakov method

Measuring Water Potential by the Gravimetric Technique. Change in weight of potato cores incubated in sucrose solutions. If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it.


Osmosis diffusion of water through a selectively permeable membrane Membrane X is permeable to water but not to protein Which side has the highest concentration.

What is the water potential of potato tissue? This is due to incipient plasmolysis that occurs at low solution water potential. A measure of how much material is packed into a unit volume of the material The fewer. Draw the best fit line for your data. Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, disperses, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly.

To make this website work, we log user data and share it with processors. The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i. Water potential values determined by this method may be slightly more negative than those obtained by the Chardokov method.

Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. In both techniques, tissue samples are incubated chardaov a series of solutions of known osmotic water potential.

Record your data in Table 2. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.


Use the following equation to calculate the percent change in weight for each tissue by the following equation: This occurs when the apoplast becomes infiltrated with water and solutes. In this method, tissue samples are weighed before and after incubation in a series of solutions of known osmotic water potential.

In contrast to the Chardakov method which analyzes changes in chardaiov density after incubation, this technique monitors tissue weight changes.

This increases the tissue weight methor may lead to small errors. Feedback Privacy Policy Feedback. Which is more accurate? Be sure not to include any fragments of the skin. Pour off the solutions into a set of empty, correspondingly labeled tubes. The answer will be in Jm -3 which are equivalent to pressure Pa. What is the water potential of potato tissue?

Chardakkov would happen to The size of the tissue? Mix the tubes thoroughly with a vortex mixer.

If you wish to chagdakov it, please recommend it to your friends in any social system. Then remove the tissues, gently blot on paper towels and reweigh. Record your results Table 2 and repeat this procedure for each of the sucrose solutions.