Horizon Scanning Technology. Prioritising Summary. LifePort. ® kidney transporter: A portable donor kidney transporter/ perfuser. November 24 – What to do after pumping begins. 28 – Removing a kidney from LifePort Kidney Transporter; removing used Perfusion Circuit after a case. 34 – 45 . The LifePort Kidney Transporter is a revolutionary method of transporting kidneys for transplantation: it is a portable, insulated perfusion transporter with.

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However, the pulsatile perfusion and size of the piping system is not suitable for rat livers. B-side was the shunt side for decreasing the portal inflow and avoiding termination of LifePort due to excessive pressure under Prime mode.

HMP, hypothermic machine perfusion. National Center for Biotechnology InformationU. Our Website uses cookies to improve your experience. Nowadays, it has trnasporter demonstrated that cold storage, a traditional organ preservation technique, cannot meet the demands of ECD preservation due tfansporter the reduced ischemic tolerance of these marginal grafts 78.

The system consisted of a rat liver container for perfusion that was installed in the sterile drape of the LifePort Kidney Transporter 1. Longterm results of liver transplantation from donation ,idney circulatory death.

Experience with liver and kidney allografts from non-heart-beating donors.

Perfusate samples from the portal inflow and the catheter cannulated in suprahepatic vena cava were respectively collected and measured using a pH-blood gas analyzer i-STAT; Abbott Point of Care, Inc. Please review our privacy policy. Assessment for the liver during HMP at 0, 3 and 6 h. In previous studies, the HMP system for rat livers typically contained a roller pump, liver container, a bubbler to deliver air, a flowmeter to measure flow, a nylon filter to prevent the recirculation of cellular debris and blood clots, a water column to measure the perfusion pressure, and a cooling system to maintain the hypothermic condition for perfusion 1221 The levels of ALT at 0, 3 and 6 h were These findings were in accordance with those observed previously in similar recirculating perfusion devices 1225 and indicated the shear stress and pressure injury of the present HMP system was minimal, and that the increased ALT and LDH release was likely due to cold ischemia injury.


The protective mechanisms for liver preservation associated with hypothermic machine perfusion HMP remain unclear. The dynamics inflow, pressure and intrahepatic resistance of perfusion were assessed to identify whether this system met the demands for HMP of rat livers.

Role of oxygen during hypothermic machine perfusion preservation of the liver. Once the ikdney was primed, the liver was connected and ready for perfusion under prime mode.

An electronic scale was installed under the liver container to calculate the portal inflow according to the association with weight, density and trsnsporter of the perfusate. In addition, the maintained hepatic OC levels, and ATP and bile production observed in the present study, which were consistent with a previous study using a similar HMP system 12suggested that the cellular metabolic activity was effectively preserved following HMP preservation for 6 h.

The Lifeport Kidney Transporter

Prevention of excessive perfusion pressure is necessary to protect organs in a LifePort transporter. By contrast, according to the preliminary results in the present study, the prime mode of Lifeport could achieve continuous perfusion, which was indicated by the oscillogram with a high frequency and small wave range. Support Center Support Center.

Functionality and use of design The LifePort kidney Transporter gently perfuses kidneys with cold physiologic solution to improve orden condition during transport to transplant recipient. In conclusion, the present study demonstrated the feasibility of a modified HMP system using a LifePort Kidney Transporter for rat liver preservation.

Cold preservation of fatty liver grafts: OC, ATP production and bile production were markers to assess cellular metabolic activity. Reviewing the impact of determining factors. Fluorescent dyes for cell viability: Division of liver and method for obtaining samples.

Introduction To overcome the shortage of brain-dead donors for liver transplantation, extended criteria donors ECDs have been used 1. Liver samples were analyzed using hematoxylin and eosin staining Fig.


The images were analyzed using the point-counting method within grids that contained 80 points combined with ImageJ 1. The lack of effective integration of these equipment was the major obstacle associated with the portability of HMP, leading to restriction for its implementation. The primary concern in the use of the LifePort for HMP preservation of rat liver is that the size of the infusion line is not suitable for the rat portal vein. Hepatic effluent was collected from the catheter cannulated in the suprahepatic vena cava every 3 h of the 6-h HMP period and the levels of alanine transaminase ALT and lactate dehydrogenase LDH were analyzed.

An application on prefixed conditions. Another poly ethylene catheter outer diameter, 3. Continuous HMP via the portal vein for liver preservation is capable of simulating hemodynamics of the portal vein and has been widely applied in a number of previous studies 1221 Assessment of islet cell viability using fluorescent dyes. Similarly, histological observations indicated the general morphology of the parenchyma was preserved and sinusoidal dilatation was significantly increased in post-HMP tissues compared with pre-HMP tissues.


At the end of hypothermic machine perfusion, samples at points a, b, c and d of CR and a’, b’, c’ and d’ points of PR were obtained. Results indicated that the levels of liefport at 0, 3 and lifepot h were 0.

H; Nanjing Jiancheng Bioengineering Institute in accordance with the manufacturer’s protocol. Subsequently, the portal inflow per min was calculated according to the association with the volume, weight and density of perfusate.

Received Dec 22; Accepted Jul C-side was connected to the infuse line and A-side was connected to lifepport pressoreceptor. Conceptual and practical considerations. Delivery of the bioactive gas hydrogen sulfide during cold preservation of rat liver: Research and need The product addresses the serious need for donation organs.